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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
25/11/2021 |
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Data da última atualização: |
25/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. S.; MARQUES, S. C. S.; GUEDES, P. H. E.; FEUCHARD, V. L.; CAMARGO, A. J. R.; FREITAS, C. de; CAMARGO, L. S. de A. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; SHEILA C. S. MARQUES; PEDRO H. E. GUEDES; VIVIANE L. FEUCHARD, Pesagro; AGOSTINHO J. R. CAMARGO, Pesagro; CELIO DE FREITAS, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Thermal-treatment protocol to induce thermotolerance in bovine embryos. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 33, n. 7, p. 497-501, 2021. |
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DOI: |
https://doi.org/10.1071/RD20309 |
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Idioma: |
Inglês |
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Conteúdo: |
Artificial reproduction in dairy cattle is challenged by summer temperatures in tropical environments. We describe a treatment based on mild temperature increasesto induce thermotolerance and improve the embryo?s performance under heat stress conditions. A protocol was established to induce upregulation of heat shock protein A (HSPA, formerly known as HSP70) but not impair embryonic development. Thermal treatment(TT) had no effect onmorula/blastocyst rate or blastocyst quality (cell number and apoptosis). Heatshock given one day after TTrevealed higher(P ¼ 0.00)survivalratesin TT blastocysts compared with Control. Treated embryos were transferred to recipients and no detrimental effects were observed regarding pregnancy rates, length, fetal growth or calf weight. Our results demonstrated that the established TT protocol could induce a thermal response by the embryo and is safe for further development. |
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Palavras-Chave: |
Choque térmico; Embryo survival; Estresse térmico; HSP; IVF; IVP; Sobrevivência embrionária. |
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Thesagro: |
Bovino. |
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Thesaurus Nal: |
Heat shock response; Thermal stress. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01852naa a2200325 a 4500
001 2136546
005 2021-11-25
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1071/RD20309$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aThermal-treatment protocol to induce thermotolerance in bovine embryos.$h[electronic resource]
260 $c2021
520 $aArtificial reproduction in dairy cattle is challenged by summer temperatures in tropical environments. We describe a treatment based on mild temperature increasesto induce thermotolerance and improve the embryo?s performance under heat stress conditions. A protocol was established to induce upregulation of heat shock protein A (HSPA, formerly known as HSP70) but not impair embryonic development. Thermal treatment(TT) had no effect onmorula/blastocyst rate or blastocyst quality (cell number and apoptosis). Heatshock given one day after TTrevealed higher(P ¼ 0.00)survivalratesin TT blastocysts compared with Control. Treated embryos were transferred to recipients and no detrimental effects were observed regarding pregnancy rates, length, fetal growth or calf weight. Our results demonstrated that the established TT protocol could induce a thermal response by the embryo and is safe for further development.
650 $aHeat shock response
650 $aThermal stress
650 $aBovino
653 $aChoque térmico
653 $aEmbryo survival
653 $aEstresse térmico
653 $aHSP
653 $aIVF
653 $aIVP
653 $aSobrevivência embrionária
700 1 $aMARQUES, S. C. S.
700 1 $aGUEDES, P. H. E.
700 1 $aFEUCHARD, V. L.
700 1 $aCAMARGO, A. J. R.
700 1 $aFREITAS, C. de
700 1 $aCAMARGO, L. S. de A.
773 $tReproduction, Fertility and Development$gv. 33, n. 7, p. 497-501, 2021.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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Data corrente: |
04/11/2022 |
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Data da última atualização: |
07/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BRUSCADIN, J. J.; CARDOSO, T. F.; DINIZ, W. J. da S.; AFONSO, J.; SOUZA, M. M. de; PETRINI, J.; ANDRADE, B. G. N.; SILVA, V. H. da; FERRAZ, J. B. S.; ZERLOTINI NETO, A.; MOURÃO, G. B.; COUTINHO, L. L.; REGITANO, L. C. de A. |
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Afiliação: |
JENNIFER JESSICA BRUSCADIN, UNIVERSIDADE FEDERAL DE SÃO CARLOS; TAINÃ FIGUEIREDO CARDOSO; WELLISON JARLES DA SILVA DINIZ, AUBURN UNIVERSITY; JULIANA AFONSO; MARCELA MARIA DE SOUZA, IOWA STATE UNIVERSITY; JULIANA PETRINI, AUBURN UNIVERSITY; BRUNO GABRIEL NASCIMENTO ANDRADE, MUNSTER TECHNOLOGICAL UNIVERSITY; VINICIUS HENRIQUE DA SILVA, UNIVERSIDADE DE SÃO PAULO; JOSÉ BENTO STERMAN FERRAZ, UNIVERSIDADE DE SÃO PAULO; ADHEMAR ZERLOTINI NETO, CNPTIA; GERSON BARRETO MOURÃO, UNIVERSIDADE DE SÃO PAULO; LUIZ LEHMANN COUTINHO, UNIVERSIDADE DE SÃO PAULO; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
Allele-specific expression reveals functional SNPs affecting muscle-related genes in bovine. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biochimica et Biophysica Acta. Gene Regulatory Mechanisms, v. 1865, n. 8, 194886, Nov. 2022. |
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DOI: |
https://doi.org/10.1016/j.bbagrm.2022.194886 |
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Idioma: |
Inglês |
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Conteúdo: |
Single nucleotide polymorphisms showing allele-specific expression (ASE SNPs) are useful for cis-regulatory variants discovery. Despite this potential, there are expensive costs involved in genome-level ASE analysis for large sample sizes. If different data resolutions are available, genotype imputation can be used to mitigate this limitation. Aiming to increase the power to detect regulatory variants, we used a large dataset (>4 million) of imputed SNP genotypes and RNA-Seq data from 190 Nelore steers. Differences between major and minor allele expressions in muscle were tested with a Binomial Test. We identified 38,177 ASE SNPs (FDR ≤ 0.05) within 7304 linkage disequilibrium blocks. After that, we searched for aseQTLs (i.e., neighboring SNPs potentially regulating the ASE SNPs' allelic expression) by comparing the ASE of heterozygous to homozygous sample groups under a Wilcoxon Rank Sum test. We identified 21,543 aseQTLs potentially regulating 430 ASE SNPs (FDR ≤ 0.05). A total of 3333 cis-eQTLs (being 2098 ASE SNPs and 1075 aseQTLs) were associated with the expression of 758 transcripts (FDR ≤ 0.05), demonstrating the cis-regulatory effect of these ASE SNPs and aseQTLs. Data integration showed reproducibility with previous studies in bovine ASE and genomic imprinting. Furthermore, we identified 36,756 novel ASE regions due to the imputation approach. Comparisons with epigenetics data from Functional Annotation of Animal Genomes (FAANG) suggest a regulatory potential of the ASE-related SNPs. The affected genes were enriched in metabolic pathways essential for muscle homeostasis. These findings reinforce the potential of using ASE for discovering cis-regulatory SNPs that may affect muscle-related traits. MenosSingle nucleotide polymorphisms showing allele-specific expression (ASE SNPs) are useful for cis-regulatory variants discovery. Despite this potential, there are expensive costs involved in genome-level ASE analysis for large sample sizes. If different data resolutions are available, genotype imputation can be used to mitigate this limitation. Aiming to increase the power to detect regulatory variants, we used a large dataset (>4 million) of imputed SNP genotypes and RNA-Seq data from 190 Nelore steers. Differences between major and minor allele expressions in muscle were tested with a Binomial Test. We identified 38,177 ASE SNPs (FDR ≤ 0.05) within 7304 linkage disequilibrium blocks. After that, we searched for aseQTLs (i.e., neighboring SNPs potentially regulating the ASE SNPs' allelic expression) by comparing the ASE of heterozygous to homozygous sample groups under a Wilcoxon Rank Sum test. We identified 21,543 aseQTLs potentially regulating 430 ASE SNPs (FDR ≤ 0.05). A total of 3333 cis-eQTLs (being 2098 ASE SNPs and 1075 aseQTLs) were associated with the expression of 758 transcripts (FDR ≤ 0.05), demonstrating the cis-regulatory effect of these ASE SNPs and aseQTLs. Data integration showed reproducibility with previous studies in bovine ASE and genomic imprinting. Furthermore, we identified 36,756 novel ASE regions due to the imputation approach. Comparisons with epigenetics data from Functional Annotation of Animal Genomes (FAANG) suggest a regulato... Mostrar Tudo |
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Palavras-Chave: |
Cis-regulação; Cis-regulation; Data integration; Epigenética; Expressão gênica; Genômica; Integração de dados; Polimorfismos de nucleotídeo único. |
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Thesagro: |
Gado. |
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Thesaurus NAL: |
Cattle; Epigenetics; Genomics; Single nucleotide polymorphism. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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Marc: |
LEADER 03063naa a2200433 a 4500
001 2148053
005 2022-11-07
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.bbagrm.2022.194886$2DOI
100 1 $aBRUSCADIN, J. J.
245 $aAllele-specific expression reveals functional SNPs affecting muscle-related genes in bovine.$h[electronic resource]
260 $c2022
520 $aSingle nucleotide polymorphisms showing allele-specific expression (ASE SNPs) are useful for cis-regulatory variants discovery. Despite this potential, there are expensive costs involved in genome-level ASE analysis for large sample sizes. If different data resolutions are available, genotype imputation can be used to mitigate this limitation. Aiming to increase the power to detect regulatory variants, we used a large dataset (>4 million) of imputed SNP genotypes and RNA-Seq data from 190 Nelore steers. Differences between major and minor allele expressions in muscle were tested with a Binomial Test. We identified 38,177 ASE SNPs (FDR ≤ 0.05) within 7304 linkage disequilibrium blocks. After that, we searched for aseQTLs (i.e., neighboring SNPs potentially regulating the ASE SNPs' allelic expression) by comparing the ASE of heterozygous to homozygous sample groups under a Wilcoxon Rank Sum test. We identified 21,543 aseQTLs potentially regulating 430 ASE SNPs (FDR ≤ 0.05). A total of 3333 cis-eQTLs (being 2098 ASE SNPs and 1075 aseQTLs) were associated with the expression of 758 transcripts (FDR ≤ 0.05), demonstrating the cis-regulatory effect of these ASE SNPs and aseQTLs. Data integration showed reproducibility with previous studies in bovine ASE and genomic imprinting. Furthermore, we identified 36,756 novel ASE regions due to the imputation approach. Comparisons with epigenetics data from Functional Annotation of Animal Genomes (FAANG) suggest a regulatory potential of the ASE-related SNPs. The affected genes were enriched in metabolic pathways essential for muscle homeostasis. These findings reinforce the potential of using ASE for discovering cis-regulatory SNPs that may affect muscle-related traits.
650 $aCattle
650 $aEpigenetics
650 $aGenomics
650 $aSingle nucleotide polymorphism
650 $aGado
653 $aCis-regulação
653 $aCis-regulation
653 $aData integration
653 $aEpigenética
653 $aExpressão gênica
653 $aGenômica
653 $aIntegração de dados
653 $aPolimorfismos de nucleotídeo único
700 1 $aCARDOSO, T. F.
700 1 $aDINIZ, W. J. da S.
700 1 $aAFONSO, J.
700 1 $aSOUZA, M. M. de
700 1 $aPETRINI, J.
700 1 $aANDRADE, B. G. N.
700 1 $aSILVA, V. H. da
700 1 $aFERRAZ, J. B. S.
700 1 $aZERLOTINI NETO, A.
700 1 $aMOURÃO, G. B.
700 1 $aCOUTINHO, L. L.
700 1 $aREGITANO, L. C. de A.
773 $tBiochimica et Biophysica Acta. Gene Regulatory Mechanisms$gv. 1865, n. 8, 194886, Nov. 2022.
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Embrapa Agricultura Digital (CNPTIA) |
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